Adaptation of the Berthelot Color Reaction for the Determination of Urea Nitrogen in Serum and Urine to an Ultramicro System.
نویسنده
چکیده
منابع مشابه
Ultramicro Methods in Biochemistry. Vii. the Determination of Plasma or Serum Urea Nitrogen. 8. the Determination of Serum Uric Acid. Ix. the Determination of Serum Creatinine. Lf-22.
Ultramicro methods for the determination of serum urea nitrogen, uric acid, and creatinine have been evaluated and the results are re corded in reports VII, VIII, and IX, respectively. The method for the determination of urea nitrogen is an ultramicro adaptation of the reaction in which a blue color is developed when the ammonium ion reacts with phenol and hypochlorite. Ammonia is released from...
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A simple method is described for directly determining serum urea nitrogen, wtihout deproteinization. The urea in 20 Ml of serum or plasma is reacted with diacetylmonoxime in the presence of thiosemicarbazide and cadmium ion under acid conditions. The absorbance of the resulting rose-purple solution is measured at 540 nm. Results agreed excellently with those obtained by a reference method that ...
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In a previous paper,3) a colorimetric method for the determination of urea nitrogen in blood and urine was presented on the basis of a color reaction of urea with diacetyl monoxime (DAM) in phosphoric acid solution in the presence of D-glucuronolactone (GL), which served to produce a photo-stable color and eliminate the sigmoidal nature of calibration curve observed usually in the DAM method.4)...
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 10 شماره
صفحات -
تاریخ انتشار 1964